| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 2221 | Leptospira biflexa | TGGGCAGCCATACAAAGGTC | CCAATCGAAACGCTGCCA | 60.32 | 58.74 | 172 | 68 |
100.00%
|
100.00%
|
| Primer 2222 | Leptospira biflexa | ACTCATTGCCCAATCGCCA | GGCCAAGGTGCACCATTTC | 60.00 | 59.71 | 258 | 68 |
100.00%
|
100.00%
|
| Primer 2223 | Leptospira biflexa | TGGCAATTCCCACAAGGTG | ACATTCATCGAGCCCACCA | 58.26 | 59.01 | 231 | 68 |
100.00%
|
100.00%
|
| Primer 2224 | Leptospira biflexa | GTTGCCATCGGAGCTTTCG | TGCTTCGTATCCTTCCGCA | 59.57 | 59.11 | 156 | 68 |
100.00%
|
100.00%
|
| Primer 2225 | Leptospira biflexa | ACTCCTGTCCGTAAGCCGT | ATTGTCGTGCTTCCCGTGT | 60.61 | 59.93 | 260 | 68 |
100.00%
|
100.00%
|
| Primer 2226 | Leptospira biflexa | ACTCCTGTCCGTAAGCCGT | GCCATCCCACATCCGAACA | 60.61 | 60.08 | 285 | 68 |
100.00%
|
100.00%
|
| Primer 2227 | Leptospira biflexa | ACTCCTGTCCGTAAGCCGT | TGCCATCCCACATCCGAAC | 60.61 | 60.08 | 286 | 68 |
100.00%
|
100.00%
|
| Primer 2228 | Leptospira biflexa | ACTCCTGTCCGTAAGCCGT | TTGTCGTGCTTCCCGTGTT | 60.61 | 60.15 | 259 | 68 |
100.00%
|
100.00%
|
| Primer 2229 | Leptospira biflexa | AGTCACTGCTGAACGCCA | TTCCCTGTTGTCAGCTGCC | 59.18 | 60.23 | 163 | 68 |
100.00%
|
100.00%
|
| Primer 2230 | Leptospira biflexa | AGTCACTGCTGAACGCCA | CCCTGTTGTCAGCTGCCAT | 59.18 | 60.30 | 161 | 68 |
100.00%
|
100.00%
|